Service
Flow Cytometry (FACS)
We provide professional experimental consulting services, from experimental design to data analysis. Through optimal model selection and customized protocol development, we enhance research efficiency and ensure reliable outcomes.
FACS Analysis (FACS Analysis Service)
The FACS Facility provides instrumentation, education and expertise for all your flow cytometry needs. The flow cytometry services include both, multi-parameter analysis and sorting of cell populations based on fluorescence labels and on phenotypic and biochemical properties of the cells.
Introduction to Flow Cytometry
- Flow cytometry is a powerful technique for multi-parameter analysis, enabling the detection of various proteins expressed in individual cells using fluorescent antibodies.
- Each cell or particle is analyzed as it rapidly passes through lasers, based on visible light scattering and fluorescence signals.
- Fluorescence can be measured through: Transcription and expression of fluorescent proteins (e.g., GFP) Antibody labeling or staining with fluorescent dyes
- Flow cytometry is widely used in research fields such as disease modeling, cancer biology, immunology, and drug development, making it a highly versatile and robust technology.
Multi-Color Panel design
- Depending on the target cell population, marker selection is determined for analysis.
- Panel design involves selecting appropriate antigens and combining them with fluorochromes, considering expression levels of markers in each cell type.
- Choosing the right fluorochrome is critical for achieving optimal resolution in FACS experiments.
- We provide customized panel design services to help you obtain the most accurate and high-quality research results.
Flow Cytometry Panel Design Consulting
- Marker combinations tailored to your research goals
- Fluorochrome recommendations based on available cytometer configurations
- Consultation on pilot experiments and gating strategies
- Pre-review protocol and customized panel sheet preparation
BD FACSCanto II
~ 8 fluorochrome parameter
BD FACSymphony A3
~ 21 fluorochrome parameter
Immune cell Phenotypic Analysis
Cell fraction measurement
- Example >
Naïve / Effector / Memory / Exhausted T cell
-
In tumor tissue
-
In draining lymph node
Immune cell Functional Analysis
Intracellular measurement
Cytokines / Transcription Factors / …
-
In tumor tissue
- Example >
Granzyme B, Ki67, TNF staining in TIL
-
- Example >
FoxP3 staining in TIL
Cytometric Beads Array
- Various cytokines can be analyzed at once in one sample.
- Detection of Pg level cytokines with small amount of sample.
The FACS Facility provides instrumentation, education and expertise for all your flow cytometry needs. The flow cytometry services include both, multi-parameter analysis and sorting of cell populations based on fluorescence labels and on phenotypic and biochemical properties of the cells.
Introduction to Flow Cytometry
- Flow cytometry is a powerful technique for multi-parameter analysis, enabling the detection of various proteins expressed in individual cells using fluorescent antibodies.
- Each cell or particle is analyzed as it rapidly passes through lasers, based on visible light scattering and fluorescence signals.
- Fluorescence can be measured through: Transcription and expression of fluorescent proteins (e.g., GFP) Antibody labeling or staining with fluorescent dyes
- Flow cytometry is widely used in research fields such as disease modeling, cancer biology, immunology, and drug development, making it a highly versatile and robust technology.
Multi-Color Panel design
- Depending on the target cell population, marker selection is determined for analysis.
- Panel design involves selecting appropriate antigens and combining them with fluorochromes, considering expression levels of markers in each cell type.
- Choosing the right fluorochrome is critical for achieving optimal resolution in FACS experiments.
- We provide customized panel design services to help you obtain the most accurate and high-quality research results.
Flow Cytometry Panel Design Consulting
- Marker combinations tailored to your research goals
- Fluorochrome recommendations based on available cytometer configurations
- Consultation on pilot experiments and gating strategies
- Pre-review protocol and customized panel sheet preparation
BD FACSCanto II
~ 8 fluorochrome parameter
BD FACSymphony A3
~ 21 fluorochrome parameter
Immune cell Phenotypic Analysis
Cell fraction measurement- Example >
Naïve / Effector / Memory / Exhausted T cell
-
In tumor tissue
-
In draining lymph node
Immune cell Functional Analysis
Intracellular measurementCytokines / Transcription Factors / …
-
In tumor tissue
- Example >
Granzyme B, Ki67, TNF staining in TIL -
- Example >
FoxP3 staining in TIL
Cytometric Beads Array
- Various cytokines can be analyzed at once in one sample.
- Detection of Pg level cytokines with small amount of sample.